CORRELATION BETWEEN AIDS-RELATED KAPOSI SARCOMA HISTOLOGICAL GRADE AND IN VITRO BEHAVIOR: REDUCED EXOGENOUS GROWTH FACTOR REQUIREMENTS FOR ISOLATES FROM HIGH GRADE LESIONS
- RT Bailer
- A Lazo
- CL Ng-Bautista
- BL Hout
- GM Ness
- AK Hegtvedt
- LE Lantry
- JR Blakeslee
- RE Stephens
- GP Brierley
- SR Mallery
Abstract
Kaposi sarcoma, the most common AIDS associated malignancy, affects 10-30% of all AIDS patients. To date, research into the biological characteristics of AIDS-related Kaposi sarcoma (AIDS-KS) derived cell lines has been based on cultures established from skin explants or pleural effusions/peritoneal fluids. We have established several AIDS-KS lines from biopsy confirmed oral mucosal and epidermal AIDS-KS lesions and have found a correlation between AIDS-KS lesional grade and in vitro cellular growth characteristics. In comparison to epidermal AIDS-KS lesions, mucosal AIDS-KS lesions frequently possessed both a more advanced histologic grade and demonstrated a greater capacity to proliferate in minimal medium. We report the ability of AIDS-KS isolates from high grade lesions to sustain proliferation (greater than 60 population doubling levels) in medium not supplemented with endothelial cell growth supplement and/or cytokine rich conditioned medium.These findings indicate that AIDS-KS cells isolated from high grade lesions have reduced requirements for exogenously provided growth supplements, and suggest that increased autologous cytokine production accompanies AIDS-KS lesional progression.
How to Cite:
Bailer, R., Lazo, A., Ng-Bautista, C., Hout, B., Ness, G., Hegtvedt, A., Lantry, L., Blakeslee, J., Stephens, R., Brierley, G. & Mallery, S., (1995) “CORRELATION BETWEEN AIDS-RELATED KAPOSI SARCOMA HISTOLOGICAL GRADE AND IN VITRO BEHAVIOR: REDUCED EXOGENOUS GROWTH FACTOR REQUIREMENTS FOR ISOLATES FROM HIGH GRADE LESIONS”, Lymphology 28(3), 126-137.
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