Articles
Authors: G Szabo ( ) , Z Magyar ( )
Urine, renal and thoracic duct lymph were collected in dogs before and after clamping both renal arteries. After 30 minutes of renal ischaemia there was a marked increase in concentration of cyto plasmic enzymes in renal lymph and urine. Less prominent rises also were observed in plasma and thoracic duct lymph.
After 2 hours of ischaemia in addition to further increase in concentration of cytoplasmic enzymes a rise in activities of mitochondrial (GPT, alc. phosphatase, arylamidase) and lysosomal (acid phosphatase) enzyines was observed in urine and renal lymph. In some animals a significant arterial-renal venous difference was found in LDH concentration, and in renal lymph the LDH isoenzyme pattern shifted towards more slowly migrating M subunits.
There was marked parallelism between enzyme concentrations in renal lymph and urine.
It is concluded, that determination of intracellular enzymes in renal lymph and urine affords a method to assess severity of ischaemic cell damage in the kidney.
Keywords:
How to Cite: Szabo, G. & Magyar, Z. (1974) “INTRACELLULAR ENZYMES IN SERUM, LYMPH AND URINE AFTER RENAL ISCHAEMIA”, Lymphology. 7(1).