TY - JOUR AB - <p>To determine whether the polyclonal immunomodulator, Phytohemagglutinin (PHA), stimulates murine lymphocytes in vivo and causes cytotoxic differentiation, we studied blastogenesis and the concomitant synthesis of macromolecules (DNA, RNA and protein) by lymphocytes at different hours after injection of PHA in mice. <sup>51</sup>Cr release assay was also performed by using allogeneic cells as target to determine whether lymphocytes after stimulation in vivo by this activator undergo cytotoxic differentiation. Out of the five doses of Phytohemagglutinin (2.5 μg, 5 μg, 10 μg, 20 μg and 50 μg per mouse) a marginally higher peak of blastogenesis was observed with 20 μg dose at 48hr. No significant peak was observed in synthesis of macromolecules or with cytotoxicity.</p> AU - TK Chaudhuri, AK Chakravarty DA - 1986/7// IS - 3 VL - 19 PB - International Society of Lymphology and the University of Arizona Libraries PY - 1986 TI - ACTIVATION OF MURINE THYMOCYTES IN VIVO: PART II: STUDY OF BLASTOGENESIS, THE SYNTHESIS OF MACROMOLECULES AND THE CYTOTOXIC RESPONSE AFTER STIMULATION WITH PHYTOHEMAGGLUTININ T2 - Lymphology UR - http://journals.librarypublishing.arizona.edu/lymph/article/id/3958/ ER -