MODULATION OF MOUSE SPLEEN NATURAL KILLER (NK) CELL ACTIVITY BY BETA-INTERFERON, INTERLEUKIN-1, AND PROSTAGLANDINS
- CL Voiculescu
- L Rosu
- S Rogoz
By using CBA/J mice as a source of effector cells and Yac-1 lymphoma line as "target" cells, the natural killer (NK) cell activity was assayed following both in vivo and in vitro immunomodulation [beta-interferon (IF), interleukin-1 (IL-1), indomethacin (IND), prostaglandin-E2 (PGE)].
Only IF/IND and IL-1/IND mixed in vivo led to a significant augmentation of NK cell activity. If exposed in vitro to IF or to IL-1, control group-derived spleen NK cells exhibited increased cytotoxic activity whereas PGE-exposure only was followed by a lower cytotoxic level as expressed both in % cytotoxicity curves and in lytic units 20%/107 effector cells.
On the other hand, PGE seemed to activate the "nonspecific suppressor" (NSS) cell subset in its inhibitory effect about NK cells as tested in vitro in several NK/NSS cell mixtures at different ratios. IF, but not IL-1 diminished the NSS-cell-induced suppressive activity. Pre-exposure of NK/NSS cell mixtures to IF followed by PGE exposure, did not prevent PGE-dependent NSS cell activation.
How to Cite:
Voiculescu, C. & Rosu, L. & Rogoz, S., (1988) “MODULATION OF MOUSE SPLEEN NATURAL KILLER (NK) CELL ACTIVITY BY BETA-INTERFERON, INTERLEUKIN-1, AND PROSTAGLANDINS”, Lymphology 21(3), 144-151.