Articles

EFFECT OF TUMOR NECROSIS FACTOR-α AND INTERLEUKIN-2 ON SPLEEN LYMPHOCYTE MIGRATION IN MOUSE SKIN

Authors: J Sun ( ) , Z Liu ( ) , Y Bi ( ) , Z Guo ( ) , T Hua ( ) , Z Ding ( )

Abstract

Tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) are reported to enhance lymphocyte binding to endothelial cells in vitro. We examined these two agents on lymphocyte migration in vivo. Spleen lymphocytes were radiolabeled with tritiated uridine (3H-UR) and then injected IV into mice. Each cytokine (TNF-α or IL-2) or both cytokines were then injected intradermally on the back of mice. The results demonstrated that TNF-α stimulates lymphocyte migration in vivo in dose-dependent fashion. Kinetic analysis demonstrated that migration with TNF-α started at 3h, peaked at 6h, followed by a gradual decline back to baseline at 24h. IL-2, on the other hand, was nearly inactive, and did not augment lymphocyte migration over and above that induced by TNF-α when both cytokines were injected together.

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How to Cite: Sun, J. , Liu, Z. , Bi, Y. , Guo, Z. , Hua, T. & Ding, Z. (1999) “EFFECT OF TUMOR NECROSIS FACTOR-α AND INTERLEUKIN-2 ON SPLEEN LYMPHOCYTE MIGRATION IN MOUSE SKIN”, Lymphology. 32(4).