Articles

CORRELATION BETWEEN AIDS-RELATED KAPOSI SARCOMA HISTOLOGICAL GRADE AND IN VITRO BEHAVIOR: REDUCED EXOGENOUS GROWTH FACTOR REQUIREMENTS FOR ISOLATES FROM HIGH GRADE LESIONS

Authors: RT Bailer ( ) , A Lazo ( ) , CL Ng-Bautista ( ) , BL Hout ( ) , GM Ness ( ) , AK Hegtvedt ( ) , LE Lantry ( ) , JR Blakeslee ( ) , RE Stephens ( ) , GP Brierley ( ) , SR Mallery ( )

Abstract

Kaposi sarcoma, the most common AIDS associated malignancy, affects 10-30% of all AIDS patients. To date, research into the biological characteristics of AIDS-related Kaposi sarcoma (AIDS-KS) derived cell lines has been based on cultures established from skin explants or pleural effusions/peritoneal fluids. We have established several AIDS-KS lines from biopsy confirmed oral mucosal and epidermal AIDS-KS lesions and have found a correlation between AIDS-KS lesional grade and in vitro cellular growth characteristics. In comparison to epidermal AIDS-KS lesions, mucosal AIDS-KS lesions frequently possessed both a more advanced histologic grade and demonstrated a greater capacity to proliferate in minimal medium. We report the ability of AIDS-KS isolates from high grade lesions to sustain proliferation (greater than 60 population doubling levels) in medium not supplemented with endothelial cell growth supplement and/or cytokine rich conditioned medium.These findings indicate that AIDS-KS cells isolated from high grade lesions have reduced requirements for exogenously provided growth supplements, and suggest that increased autologous cytokine production accompanies AIDS-KS lesional progression.

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How to Cite: Bailer, R. , Lazo, A. , Ng-Bautista, C. , Hout, B. , Ness, G. , Hegtvedt, A. , Lantry, L. , Blakeslee, J. , Stephens, R. , Brierley, G. & Mallery, S. (1995) “CORRELATION BETWEEN AIDS-RELATED KAPOSI SARCOMA HISTOLOGICAL GRADE AND IN VITRO BEHAVIOR: REDUCED EXOGENOUS GROWTH FACTOR REQUIREMENTS FOR ISOLATES FROM HIGH GRADE LESIONS”, Lymphology. 28(3).