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CORRELATION BETWEEN AIDS-RELATED KAPOSI SARCOMA HISTOLOGICAL GRADE AND IN VITRO BEHAVIOR: REDUCED EXOGENOUS GROWTH FACTOR REQUIREMENTS FOR ISOLATES FROM HIGH GRADE LESIONS

Authors
  • RT Bailer
  • A Lazo
  • CL Ng-Bautista
  • BL Hout
  • GM Ness
  • AK Hegtvedt
  • LE Lantry
  • JR Blakeslee
  • RE Stephens
  • GP Brierley
  • SR Mallery

Abstract

Kaposi sarcoma, the most common AIDS associated malignancy, affects 10-30% of all AIDS patients. To date, research into the biological characteristics of AIDS-related Kaposi sarcoma (AIDS-KS) derived cell lines has been based on cultures established from skin explants or pleural effusions/peritoneal fluids. We have established several AIDS-KS lines from biopsy confirmed oral mucosal and epidermal AIDS-KS lesions and have found a correlation between AIDS-KS lesional grade and in vitro cellular growth characteristics. In comparison to epidermal AIDS-KS lesions, mucosal AIDS-KS lesions frequently possessed both a more advanced histologic grade and demonstrated a greater capacity to proliferate in minimal medium. We report the ability of AIDS-KS isolates from high grade lesions to sustain proliferation (greater than 60 population doubling levels) in medium not supplemented with endothelial cell growth supplement and/or cytokine rich conditioned medium.These findings indicate that AIDS-KS cells isolated from high grade lesions have reduced requirements for exogenously provided growth supplements, and suggest that increased autologous cytokine production accompanies AIDS-KS lesional progression.

How to Cite:

Bailer, R. & Lazo, A. & Ng-Bautista, C. & Hout, B. & Ness, G. & Hegtvedt, A. & Lantry, L. & Blakeslee, J. & Stephens, R. & Brierley, G. & Mallery, S., (1995) “CORRELATION BETWEEN AIDS-RELATED KAPOSI SARCOMA HISTOLOGICAL GRADE AND IN VITRO BEHAVIOR: REDUCED EXOGENOUS GROWTH FACTOR REQUIREMENTS FOR ISOLATES FROM HIGH GRADE LESIONS”, Lymphology 28(3), 126-137.

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Published on
05 Sep 1995
Peer Reviewed