TY - JOUR AB - <p>The nature of Kaposi sarcoma (KS) (vascular malignancy vs. discordant angiogenesis) and lineage of the progenitor cell remain unclear. Therefore, AIDS-KS enzyme isolate cultures were prepared from excised skin lesions. Endothelial marker positivity for Factor VIII related antigen (F8RAg), Ulex europaeus agglutinin (UEA), and angiotensin-converting enzyme (ACE) were determined by fluorescence microscopy (FM) and flow cytometry (FCM). DNA cell-cycle analysis was performed using FCM. KS lesions showed large thick-walled channels (F8RAg and UEA strongly +), narrow vascular slits and thin-walled lakes (F8RAg and UEA weakly +), and non-prominent spindle cells (F8RAg and UEA almost uniformly negative). KS cultures yielded heterogenous populations of spindle, stellate, and flattened endothelial-like cells, displaying positivity for F8RAg (64 +/- 3%; mean +/- SE), UEA (40 +/- 9%), and ACE (81 +/- 9%). When injected subcutaneously in the nude mouse these cells failed to produce tumors. During contact inhibition induced quiescence, KS cultures exhibited a high G<sub>2</sub>M (18 +/- 3%) compared to non-KS (7 +/- 4%; p &lt; 0.04), evidence of an altered proliferative potential consistent with a transdifferentiated or transformed phenotype.</p> AU - DL Way, MH Witte, M Fiala, G Ramirez, RB Nagle, MJ Bernas, M Dictor, P Borgs, CL Witte DA - 1993/9// IS - 2 VL - 26 PB - International Society of Lymphology and the University of Arizona Libraries PY - 1993 TI - Endothelial transdifferentiated phenotype and cell-cycle kinetics of AIDS-associated Kaposi sarcoma cells T2 - Lymphology UR - http://journals.librarypublishing.arizona.edu/lymph/article/id/3207/ ER -