@article{lymph 3161, author = {S Kato, A Yasunaga, U Uchida}, title = {ENZYME-HISTOCHEMICAL METHOD FOR IDENTIFICATION OF LYMPHATIC CAPILLARIES}, volume = {24}, year = {1991}, url = {http://journals.librarypublishing.arizona.edu/lymph/article/id/3161/}, issue = {3}, abstract = {<p>Using 5'-nucleotidase (5'-Nase)-alkaline phosphatase (ALPase) double staining, lymphatic capillaries and blood capillaries were distinguished histochemically on cold glycol methacrylate (JB-4) sections of monkey intestines, on the basis of both enzyme characteristics. The specificity of the 5'-Nase reaction was obtained by inhibiting nonspecific ALPase in the 5'-Nase incubation medium including L-tetramisole. This double staining method demonstrated satisfactory isolated visualization of 5'-Nase activity in lymphatic capillaries and of ALPase activity in blood capillaries under light microscopy. The intensity and localization of 5'-Nase activity on the walls of lymphatic capillaries were also determined by the lead-based method under transmission electron microscopy. The intense reaction products of 5'-Nase activity were predominantly deposited on the outer surface of the plasma membrane of the lymphatic endothelial cells.</p>}, month = {10}, pages = {125-129}, issn = {2522-7963}, publisher={International Society of Lymphology and the University of Arizona Libraries}, journal = {Lymphology} }